Light chain 1 of microtubule-associated protein 1B can negatively regulate the action of Pes1.

摘要: Pes1 was first identified as the locus affected in zebrafish mutant pescadillo, which exhibits severe defects gut and liver development. It has since been demonstrated that loss of expression mammals yeast affects ribosome biogenesis, resulting a block cell proliferation. contains BRCA1 C-terminal domain, structural motif shown to facilitate protein-protein interactions, suggesting binding partners. We used two-hybrid screen identify putative interacting proteins. found light chain 1 microtubule-associated protein 1B (Mtap1b-LC1) could partner with Pes1, deletion analyses revealed specific interaction Mtap1b-LC1 domain. confirmed integrity between by co-immunoprecipitation experiments. Protein localization studies NIH3T3 cells exogenously expressed typically restricted nuclei nucleoli. However, exogenous predominantly cytoplasm were forced express Mtap1b-LC1. also observed endogenous significantly reduced or undetectable when overexpressed, implying dynamic exists two proteins potential negatively impact function. Finally, we that, is case depleted shRNA, overexpression resulted diminished proliferation cells, repress modulating nucleolar levels Pes1.