Simulated microgravity affects ciprofloxacin susceptibility and expression of acrAB-tolC genes in E. coli ATCC25922.
作者： Yuling Huang , Chenglin Li , Jianzhong Zhang , Yan Cui , Yanhua Zheng
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摘要: As a representative fluoroquinolone antibacterial, ciprofloxacin is frequently used to treat infections caused by bacteria such as E. coli. It much meaningful explore susceptibility and investigate possible mechanism of drug changes in coli ATCC25922 exposed the environmental stress simulated microgravity. The subculture lasted for 7 days under microgravity conditions (SMG) normal (NG) conditions. On 8th day, cultures were divided into three groups: (1) NG group (continuous cultures); (2) SMG (3) SMCNG (simulated change gravity cultures). Ciprofloxacin (a final concentration 0.125 μg/ml) sensitivity expression acrAB-tolC genes detected cells. count percentage viable cells higher than that reduced levels when they subcultivated from NG. expressions efflux pump (acrA, acrB tolC) upregulated culture downregulated Susceptibility could be reversibly affected Over perhaps played an important role decreased CIP SMG.
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