The effect of cyclic mechanical strain on the expression of adhesion‐related genes by periodontal ligament cells in two‐dimensional culture

摘要: Saminathan A, Vinoth KJ, Wescott DC, Pinkerton MN, Milne TJ, Cao T, Meikle MC. The effect of cyclic mechanical strain on the expression adhesion-related genes by periodontal ligament cells in two-dimensional culture. J Periodont Res 2012; 47: 212–221. © 2011 John Wiley & Sons A/S Background and Objective:  Cell adhesion plays important roles maintaining structural integrity connective tissues sensing changes biomechanical environment cells. objective present investigation was to extend our understanding human cells. Material Methods:  Cultured were subjected a in-plane tensile deformation 12% for 5 s (0.2 Hz) every 90 s 6–24 h Flexercell FX-4000 Strain Unit. following parameters measured: (i) cell viability MTT assay; (ii) caspase-3 -7 activity; (iii) the 84 encoding molecules using real-time RT-PCR microarrays. Results:  Mechanical stress reduced metabolic activity deformed at 6 h, caspase-3 6 12 h. Seventy-three detected critical threshold values < 35. Fifteen showed significant change relative expression: five cell (ICAM1, ITGA3, ITGA6, ITGA8 NCAM1), three collagen α-chains (COL6A1, COL8A1 COL11A1), four MMPs (ADAMTS1, MMP8, MMP11 MMP15), plus CTGF, SPP1 VTN. Four upregulated ICAM1 SPP1) 11 downregulated, with range extending from 1.76-fold induction 12 h 2.49-fold downregulation COL11A1 24 h. Conclusion:  study has identified several mechanoresponsive genes, shown that onset followed transient reduction overall cellular activity, including two apoptosis ‘executioner’ caspases.