HISTOCHEMICAL STAINS FOR MACROPHAGES IN CELL SMEARS AND TISSUE SECTIONS: β-GALACTOSIDASE, ACID PHOSPHATASE, NONSPECIFIC ESTERASE, SUCCINIC DEHYDROGENASE, AND CYTOCHROME OXIDASE

摘要: Publisher Summary This chapter discusses the use of histochemical stains for macrophages in cell smears and tissue sections. Macrophages with strong microbicidal activity probably have high levels mitochondrial enzymes because these are involved oxygen transport. In tubercle bacilli (BCG) granulomas, specific digestive may vary substance ingested by macrophage. other words, acid phosphatase, but not β-galactosidase, be certain macrophages, vice versa. The indolyl method is used demonstration β-galactosidase. has proved to ideal studying digestive-type activation rabbit macrophages. blue-green color resulting from β-galactosidase varies zero unactivated almost black strongly activated readily distinguishable pure blue hematoxylin counterstain. histochemistry, phosphatase classic lysosomal enzyme. lead substitution localized phagosomes can electron microscopy.