A sustainable biotechnological process for the efficient synthesis of kojibiose

摘要: This work reports the optimization of a cost-effective and scalable process for enzymatic synthesis kojibiose (2-O-α-D-glucopyranosyl-α-D-glucose) from readily available low-cost substrates such as sucrose lactose. biotechnological is based on dextransucrase-catalysed initial galactosyl-derivative (4-O-β-D-galactopyranosyl-kojibiose) followed by removal residual monosaccharides using Saccharomyces cerevisiae yeast treatment, thorough hydrolysis step with Kluyveromyces lactis β-galactosidase. Depending final purification stage, i.e. extension treatment or use preparative liquid chromatography, purity produced ranged 65% to ≥99%, respectively. The moderately high yield achieved (38%, in weight respect amount lactose) this affordable could expand potential applications according bioactive properties that have been associated disaccharide, so far limited its low availability.