Insights into the accumulation and transformation of Ch-SeNPs by Raphanus sativus and Brassica juncea: Effect on essential elements uptake

摘要: Abstract Selenium (Se) at very low doses has important functions for humans. Unfortunately, the levels of Se in soils various regions world have implemented agronomic biofortification crops by applying Se-enriched fertilizers (mainly based on selenate). Lately, use nanofertilizers is growing interest as their size reduces amount chemicals and minimizes nutrient losses comparison with conventional bulk fertilizers. However, knowledge fate environmental impact still scarce. This study aims to evaluate biotransformation chitosan-modified nanoparticles (Ch-SeNPs) well effect metabolism essential metals (Fe, Cu, Zn Mo) when applied hydroponic cultivation R. sativus B. juncea. In house-synthesized Ch-SeNPs were characterized both synthesis culture media transmission electron microscopy (TEM), dynamic light scattering (DLS) nanoparticle tracking analysis (NTA). The composition one-tenth strength Hoagland's solution did not affect size, shape concentration number particles per mL Ch-SeNPs. plants grown inside a box 25 °C during months May–July 2018. After week treatment Ch-SeNPs, harvested divided into roots aerial part. was evaluated through process enzymatic hydrolysis subsequent HPLC-ICP-MS HPLC-ESI-MS/MS. results confirmed transformation seleno-amino acids: Selenomethionine (SeMet), Semethylselenocysteine (SeMetSeCys) ɣ-glutamyl-Se-MetSeCys. Moreover, Multiple-way variance (ANOVA) principal component (PCA) showed that, regardless plant species, supplementation affected absorption Zn.