Brain protein preservation largely depends on the postmortem storage temperature: implications for study of proteins in human neurologic diseases and management of brain banks: a BrainNet Europe Study.

摘要: The present study was designed to reveal protein modifications in control cases related with postmortem delay and temperature of storage 3 paradigms which the same tissue sample (frontal cortex) frozen a short time after death or stored at 1 degrees C, 4 room then -80 C different intervals. No evidence degradation as revealed monodimensional gel electrophoresis Western blotting observed samples artificially intervals up 50 hours death. However, levels several proteins were modified this effect more marked temperature. Two-dimensional mass spectrometry further corroborated these observations permitted identification other vulnerable resistant delay. Finally, sarkosyl-insoluble fractions Alzheimer disease showed reduced intensity phospho-tau-specific bands effects being dramatic when brain for long periods. These results emphasize necessity reducing body minimize degradation.