Detection of cell cycle- and differentiation stage-dependent human telomerase reverse transcriptase expression in single living cancer cells

摘要: Elevated telomerase activity is an important molecular signature of cancer cells and primitive in regenerative tissues. However, isolation single living with endogenous has not yet been possible. Here, we developed adenovirus serotype 35 tropism-based vectors encoding destabilized enhanced green fluorescence protein a half-life 2 h (d2EGFP) driven by the human reverse transcriptase (hTERT) promoter. As assessed telomerase-positive or -negative cell lines, d2EGFP expression positively correlated hTERT transcript content activity. In retinoic acid-induced differentiating HL-60 cells, diminished same manner as expression. Individual from HeLa lines exhibited heterogeneous expression, which was cycle dependent, sorted d2EGFP+ contained twice many S/G2/M phase compared d2EGFP- cells. both populations proliferation regeneration capacities. Heterogeneous also detected xenograft glioblastoma multiforme tumor formation capacity. Thus, reported cycle- differentiation stage-dependent Our study facilitates characterization