Isolation of a highly quiescent subpopulation of primitive leukemic cells in chronic myeloid leukemia.
作者： Tessa Holyoake , Xiaoyan Jiang , Connie Eaves , Allen Eaves
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摘要: Chronic myeloid leukemia (CML) is characterized by an increased proliferative activity of the leukemic progenitors that produce an elevated number of mature granulocytes. Nevertheless, cell cycle-active agents, even in very high doses, are alone unable to eradicate the leukemic clone, suggesting the presence of a rare subset of quiescent leukemic stem cells. To isolate such cells, we first used Hoechst 33342 and Pyronin Y staining to obtain viable G0 and G1/S/G2/M fractions of CD34+ cells by fluorescence …
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