An effective method for de novo peptide sequencing based on phosphorylation strategy and mass spectrometry.
作者： Dongmei Zhang , Hongxia Liu , Shusheng Zhang , Xiaolan Chen , Shangfu Li
DOI: 10.1016/J.TALANTA.2010.12.035
关键词:
摘要: Abstract An effective method for peptide sequencing based on phosphorylation strategy and UPLC–MS/MS is proposed in this report. A reaction was carried out by mixing model solution with solution. used to analyze characterize the phosphorylated peptides optimized ramp collision energy mode. The results illustrated that approach significantly strengthened signal intensity of both a1 b series ions spectra modified peptides. It also can be effectively distinguish glutamine (Q) lysine (K) residues feasibility validated analyzing trypsin-digested BSA. Data suggested could a useful tool de novo proteome research.
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