Method for enzyme-catalysed hydrolysis of polyacrylic acid esters and esterases to be used
作者： Marlene Buchebner , Brigitte Pohn , Bernhard Prof. Dr. Hauer , Branneby Cecilia Dr. Kvarnström , Sabine Dr. Feichtenhöfer
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摘要: Method for enzyme-catalyzed hydrolysis of polyacrylic acid esters, comprises (a) providing at least one esters; (b) incubating the ester with enzyme, which is an enzyme acting on bonds, until groups contained in are partially or completely, hydrolytically cleaved; and optionally (c) isolating modified polymer. Independent claims included for: (1) a functional esterase mutant; (2) nucleic acid, codes mutant, complementary to (a), hybridized under stringent conditions, preferably such acids, show sequence identity 80%, mutant family VIII type C ester; (3) vector comprising acid; (4) microorganisms vector; (5) producing esterase, culturing host organism expressing inducing expression from and/or culture medium; (6) polymer reaction product obtained esters.
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