Fluorescence sensor for detecting protamines based on competitive interactions of polyacrylic acid modified with sodium 4-amino-1-naphthalenesulfonate with protamines and aminated graphene oxide
作者： Jinshui Liu , Meijiao Xu , Bin Wang , Zihan Zhou , Lizhen Wang
DOI: 10.1039/C6RA24793H
关键词:
摘要: An easy-to-use fluorescent probe for detecting protamines was developed. Protamines are detected using the because of competitive interactions polyacrylic acid modified with sodium 4-amino-1-naphthalenesulfonate (ANS-PAA) and aminated graphene oxide (GO-NH2). The ANS-PAA fluorescence effectively quenched by GO-NH2, high quenching efficiency being caused becoming non-covalently bound to GO-NH2 surface through electrostatic interactions, forming a ANS-PAA/GO-NH2 complex. Adding recovered This recovery due positively charged removing from surfaces ANS-PAA/protamine complexes. Displacement gave efficiency. Under optimal conditions, intensity when were added proportional protamine concentration over range 0 6.0 μg mL−1. detection limit 0.4 approach offers new quantitative method determining protamines.
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