The Kodak Ektachem clinical chemistry slide for measurement of bilirubin in newborns: principles and performance.

摘要: In this slide, unconjugated bilirubin and its sugar conjugates interact with a cationic polymeric mordant to form spectrally enhanced complexes having similar absorptivities at approximately 400 nm. With reflection densitometry appropriate mathematical transformation, readings wavelength are linearly related concentrations up 260 mg/L. The slide requires 10 microL of serum, is precise (total CV less than 2% determined over 20 days for the analyte range 39-184 mg/L), gives results that correlate well Doumas et al. modification Jendrassik-Grof method (slope 0.95, intercept 0.3, Sy . x 3.4, r = 0.991), relatively interference free. Also, measures loss after in vitro illumination serum specimens do diazo tests. An intermediate layer minimizes spectral from hemoglobin prevents detection strongly protein-linked ("delta") found many jaundiced adults. recommended newborns (less or equal 14 days), whom incidence delta negligible.