Antistreptococcal group A antibodies: production after in vitro activation and hybridization of mouse spleen cells.

摘要: Abstract Streptococcal group A polysaccharide-specific antibodies were raised by the method of somatic cell hybridization. Spleen cells experimentally unprimed BALB/c and C57BL/6 mice activated in vitro streptococcal vaccine fused with Sp2/0-Ag14 line at times 0, 35, 70, 105 h thereafter. Hybridomas obtained all independent addition thymocyte-conditioned medium. Occurrence specific hybridomas for T cell-dependent A-CHO, however, required activation greater than 35 h. Low responder splenocytes considerably higher fusion efficiency to yield high spleen after antigen. The isotypes A-CHO-specific comprised predominantly μ k polypeptides; γ3, α, λ polypeptide chains also identified. All agglutinating cells; this agglutination was fully inhibited 1 % N-acetyl-D-glucosamine, immune determinant sugar A-CHO. Three cloned grown as tumours peritoneal cavity mice. monoclonal ascites did not precipitate A-CHO but continued agglutinate a hapten inhibitable fashion different specificity profiles. Antibody from clone 21S36.1 coprecipitable upon γG3 hybridoma-derived antibody ratio sol1 7 while other two γM S117 myeloma protein not. This result suggests that recognizes one chain terminal