Intergeneric protoplast fusion between Kluyveromyces and Saccharomyces cerevisiae - to produce sorbitol from Jerusalem artichokes

摘要: The construction of inulin-assimilating and sorbitol-producing fusants was achieved by intergeneric protoplast fusion between Kluyveromyces sp. Y-85 Saccharomyces cerevisiae E-15. cells parental strains were pretreated with 0.1% EDTA (w/v) 2-mercaptoethanol (0.1%, v/v) then exposed to 2.0% Zymolase at 30 °C for 30–40 min. optimized condition demonstrated that the presence 30% polyethylene glycol 6000 (PEG-6000) 10 mM CaCl2 min, frequency reached 2.64 fusants/106 cells. screened different characters two further identified DNA contents, inulinase activity sorbitol productivity. One genetically stable fusants, Strain F27, a maximal production 4.87 g/100 ml under optimal fermentation condition.