Salient Sub-Second Separations
作者： M. Farooq Wahab , Rasangi M. Wimalasinghe , Yadi Wang , Chandan L. Barhate , Darshan C. Patel
DOI: 10.1021/ACS.ANALCHEM.6B02260
关键词:
摘要: Sub-second liquid chromatography in very short packed beds is demonstrated as a broad proof of concept for chiral, achiral, and HILIC separations biologically important molecules. Superficially porous particles (SPP, 2.7 μm) different surface chemistries, namely, teicoplanin, cyclofructan, silica, quinine, were 0.5-cm-long columns separating classes compounds. Several issues must be addressed to obtain the maximum performance 0.5 cm with reduced plate heights 2.6 3.0. Modified UHPLC hardware can used sub-second provided extra-column dispersion minimized sufficient data acquisition rates are used. Further, improvements will needed take full advantage faster separations. The utility power transform, which already employed certain detectors, shown advantageous chromatography. This approach could prove beneficial fast screening two-dimensional liq...
acs.org
core.ac.uk 
sci-hub.se 参考文章(34)
Fabrice Gritti, Irene Leonardis, Jude Abia, Georges Guiochon, Physical properties and structure of fine core–shell particles used as packing materials for chromatography Journal of Chromatography A. ,vol. 1217, pp. 3819- 3843 ,(2010) , 10.1016/J.CHROMA.2010.04.026
Alexandra Mandl, Lorenzo Nicoletti, Michael Lämmerhofer, Wolfgang Lindner, Quinine versus carbamoylated quinine-based chiral anion exchangers. A comparison regarding enantioselectivity for N-protected amino acids and other chiral acids. Journal of Chromatography A. ,vol. 858, pp. 1- 11 ,(1999) , 10.1016/S0021-9673(99)00803-1
Fabrice Gritti, Thomas McDonald, Martin Gilar, Accurate measurement of dispersion data through short and narrow tubes used in very high-pressure liquid chromatography. Journal of Chromatography A. ,vol. 1410, pp. 118- 128 ,(2015) , 10.1016/J.CHROMA.2015.07.086
Chandan L. Barhate, M. Farooq Wahab, Zachary S. Breitbach, David S. Bell, Daniel W. Armstrong, High efficiency, narrow particle size distribution, sub-2 μm based macrocyclic glycopeptide chiral stationary phases in HPLC and SFC. Analytica Chimica Acta. ,vol. 898, pp. 128- 137 ,(2015) , 10.1016/J.ACA.2015.09.048
Agnès Le Masle, David Angot, Cécile Gouin, Amélie D’Attoma, Jérémie Ponthus, Alain Quignard, Sabine Heinisch, Development of on-line comprehensive two-dimensional liquid chromatography method for the separation of biomass compounds Journal of Chromatography A. ,vol. 1340, pp. 90- 98 ,(2014) , 10.1016/J.CHROMA.2014.03.020
Natalia Piehl, Martin Ludwig, Detlev Belder, Subsecond chiral separations on a microchip Electrophoresis. ,vol. 25, pp. 3848- 3852 ,(2004) , 10.1002/ELPS.200406028
D. Shock, G.R. Dennis, G. Guiochon, P.K. Dasgupta, R.A. Shalliker, Anomalies in evaporative light scattering detection Analytica Chimica Acta. ,vol. 703, pp. 245- 249 ,(2011) , 10.1016/J.ACA.2011.06.060
Csaba G. Horvath, B. A. Preiss, Seymour R. Lipsky, Fast liquid chromatography. Investigation of operating parameters and the separation of nucleotides on pellicular ion exchangers Analytical Chemistry. ,vol. 39, pp. 1422- 1428 ,(1967) , 10.1021/AC60256A003
Szabolcs Fekete, Katalin Ganzler, Jenő Fekete, Efficiency of the new sub-2 μm core-shell (Kinetex™) column in practice, applied for small and large molecule separation. Journal of Pharmaceutical and Biomedical Analysis. ,vol. 54, pp. 482- 490 ,(2011) , 10.1016/J.JPBA.2010.09.021
Alvin W. Moore, James W. Jorgenson, Rapid comprehensive two-dimensional separations of peptides via RPLC-optically gated capillary zone electrophoresis Analytical Chemistry. ,vol. 67, pp. 3448- 3455 ,(1995) , 10.1021/AC00115A013