Neutral lipid profile of sago worm, the larva of weevil rynchophorus ferrugineus

作者: K. L. Chu , C. W. Wang , Ozair Mohd. Ali

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摘要: The larva of the weevil Rynchophorus ferrugineus is popularly known as sago worm. worms were purchased from wet market in Kuching, Sarawak. fat tissues dissected separately viscera and cuticle. neutral lipid content tissue was extracted with 150 ml dichloromethane by using a Soxhlet apparatus. per gm (fresh weight) found to be 59.8% whereas that cuticle 11.7%. profile triacylglycerols analyzed high performance liquid chromatography (Waters 501 HPLC pump) equipped evaporative light scattering detector (ELSD, VAREX MKIII). column used Supelcosil LC-18 solvent system acetonitrile /dichloromethane (68:32,v/v). identification carried out purified olive linseed oils commercially available standards. From HPLC-ELSD chromatograms, two (2) major triacylglycerols, namely triolein (OOO) l-palmitoyl-2- oleoyl-3-oleoyl-rac-glycerol (POO) at least ten (10) other minor identified. percentage (w/w) both 50% l-palmitoyl-2-oleoyl-3-oleoyl-racglycerol 41.7%. Other identified l-palmitoyl-2-oleoyl-3-linolenoyl-rac-glycerol, POLn (0.6%), 1- palmitoyl-2-linoleoyl-3-linoleoyl-rac-glycerol, PLL (0.1%), l-palmitoyl-2-palmitoyl-3- linoleoyl-rac-glycerol, PPL(1.1%) l-palmitoyl-2-stearoyl-3-linoleoyl-rac-glycerol, PSO (2.9%) (0.2%), (1.0%), (2.9%).

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