Comparison of Minocycline Susceptibility Testing Methods for Carbapenem-Resistant Acinetobacter baumannii

摘要: Treatment options for infections due to carbapenem-resistant Acinetobacter baumannii are extremely limited. Minocycline is a semisynthetic tetracycline derivative with activity against this pathogen. This study compared susceptibility testing methods that used in clinical microbiology laboratories (Etest, disk diffusion, and Sensititre broth microdilution methods) of minocycline, tigecycline, doxycycline 107 A. isolates. Susceptibility rates determined the standard method using cation-adjusted Mueller-Hinton (MH) were 77.6% minocycline 29% doxycycline, 92.5% isolates had tigecycline MICs ≤2 μg/ml. Using MH agar from BD Oxoid, Etest 67.3% 52.3% 21.5% 18.7% 71% 29.9% respectively. With diffusion 82.2% 72.9% 34.6% respectively, μg/ml 46.7% 23.4% tigecycline. In comparison results, very major low (∼2.8%) all three drugs across methods, but error higher (∼5.6%), especially method. For minor ranged 14% 37.4%. 6.5% 69.2%. The majority errors susceptible results being reported as intermediate. strains, rare, overcalling strains intermediate or resistant occur frequently feasible laboratories.