DNA Electrochemistry with Tethered Methylene Blue
作者： Catrina G. Pheeney , Jacqueline K. Barton
DOI: 10.1021/LA300566X
关键词:
摘要: Methylene blue (MB′), covalently attached to DNA through a flexible C12 alkyl linker, provides sensitive redox reporter in electrochemistry measurements. Tethered, intercalated MB′ is reduced DNA-mediated charge transport; the incorporation of single base mismatch at position 3, 10, or 14 17-mer causes an attenuation signal 62 ± 3% well-matched DNA, irrespective duplex. The intensity for MB′–DNA found be least 3-fold larger than that Nile (NB)–DNA, indicating even more strongly coupled π-stack. due intervening does, however, depend on film density and backfilling agent used passivate surface. These results highlight two mechanisms reduction DNA-modified electrode: mediated by pair stack direct surface electrode. are distinguished their rates electron transfe...
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Charge transfer in DNA : from mechanism to application Wiley-VCH. ,(2005) , 10.1002/3527606629
Elizabeth M. Boon, Donato M. Ceres, Thomas G. Drummond, Michael G. Hill, Jacqueline K. Barton, Mutation detection by electrocatalysis at DNA-modified electrodes. Nature Biotechnology. ,vol. 18, pp. 1096- 1100 ,(2000) , 10.1038/80301
Jason D. Slinker, Natalie B. Muren, Sara E. Renfrew, Jacqueline K. Barton, DNA charge transport over 34 nm Nature Chemistry. ,vol. 3, pp. 228- 233 ,(2011) , 10.1038/NCHEM.982
Francesco Ricci, Andrew J. Bonham, Aaron C. Mason, Norbert O. Reich, Kevin W. Plaxco, Reagentless, electrochemical approach for the specific detection of double- and single-stranded DNA binding proteins. Analytical Chemistry. ,vol. 81, pp. 1608- 1614 ,(2009) , 10.1021/AC802365X
Stephen J. Wagner, Andrey Skripchenko, Daniel Robinette, James W. Foley, Louis Cincotta, Factors Affecting Virus Photoinactivation by a Series of Phenothiazine Dyes Photochemistry and Photobiology. ,vol. 67, pp. 343- 349 ,(1998) , 10.1111/J.1751-1097.1998.TB05208.X
Agnès Anne, Agnès Bouchardon, Jacques Moiroux, 3‘-Ferrocene-Labeled Oligonucleotide Chains End-Tethered to Gold Electrode Surfaces: Novel Model Systems for Exploring Flexibility of Short DNA Using Cyclic Voltammetry Journal of the American Chemical Society. ,vol. 125, pp. 1112- 1113 ,(2003) , 10.1021/JA028640K
Daniel Pedersen, Christoph Rosenbohm, Dry Column Vacuum Chromatography Synthesis. ,vol. 2001, pp. 2431- 2434 ,(2004) , 10.1055/S-2001-18722
Daniel A. Di Giusto, Wjatschesslaw A. Wlassoff, Susanne Giesebrecht, J. Justin Gooding, Garry C. King, Multipotential electrochemical detection of primer extension reactions on DNA self-assembled monolayers. Journal of the American Chemical Society. ,vol. 126, pp. 4120- 4121 ,(2004) , 10.1021/JA0319036
Chunhai Fan, Xiaolei Zuo, Shiping Song, Jiong Zhang, Dun Pan, Lihua Wang, A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP. Journal of the American Chemical Society. ,vol. 129, pp. 1042- 1043 ,(2007) , 10.1021/JA067024B
Arica A. Lubin, Brook Vander Stoep Hunt, Ryan J. White, Kevin W. Plaxco, Effects of Probe Length, Probe Geometry, and Redox-Tag Placement on the Performance of the Electrochemical E-DNA Sensor Analytical Chemistry. ,vol. 81, pp. 2150- 2158 ,(2009) , 10.1021/AC802317K