Levels of the Alkoxy Radical in Patients with Brain Death
作者： Kenji Dohi , Kazue Satoh , Hiroshi Moriwaki , Yuko Mihara , Yasufumi Miyake
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摘要: Lipid peroxidation is induced by reactive oxygen species and involved in acute neuronal damage. Thus, controlling this process may be a realistic therapeutic strategy for treating or preventing However, the study of free radicals body fluids currently severely hampered technical difficulties their detection. We have developed an ex vivo electron spin resonance (ESR) method, that employs 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as trap, to measure alkoxyl radical (OR) human blood. found method can detect OR produced blood vivo. Analysis jugular venous radial arterial from patients with damage revealed higher levels compared samples. In brain death patients, however, vein spectrum was similar artery level. The ratio between levels, expressed RI (radical intensity) = aORI/jORI, calculated. A rterial-jugular bulb below 1, together accepted clinical criteria (unresponsive coma brainstem areflexia), provide non-invasive assessment cerebral circulatory arrest help predict death. This novel ESR very useful measuring oxidative stress
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