Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture.

摘要: Coronaviruses are important human and animal pathogens, the relevance of which increased due to emergence new coronaviruses like SARS-CoV, HKU1 NL63. Together with toroviruses, arteriviruses, roniviruses belong order Nidovirales. So far antivirals hardly available combat infections viruses this order. Therefore, various antiviral strategies counter nidoviral under evaluation. Lectins, bind N-linked oligosaccharide elements enveloped viruses, can be considered as a conceptionally class virus inhibitors. These agents were recently evaluated for their activity towards variety shown in most cases inhibit infection at low concentrations. However, limited knowledge is efficacy nidoviruses. In article application plant lectins Hippeastrum hybrid agglutinin (HHA), Galanthus nivalis (GNA), Cymbidium sp. (CA) Urtica dioica (UDA) well non-plant derived pradimicin-A (PRM-A) cyanovirin-N (CV-N) potential was evaluated. Three tests compared based on different evaluation principles: cell viability (MTT-based colorimetric assay), number infected cells (immunoperoxidase assay) amount viral protein expression (luciferase-based assay). The presence carbohydrate-binding strongly inhibited (transmissible gastroenteritis virus, infectious bronchitis feline serotypes I II, mouse hepatitis virus), arteriviruses (equine arteritis porcine respiratory reproductive syndrome virus) torovirus Berne virus). Remarkably, serotype II not by PRM-A, contrast other tested.