Binding characteristics of endothelin ETA receptors in normal and post-mortem rat lung

摘要: Abstract In control lung homogenates, optimal specific binding of [ 125 I]endothelin-1 and minimal filter was achieved using 50 μg/ml bacitracin, 30 μM phenylmethylsulphonyl fluoride (PMSF) 10 mM EDTA. post-mortem tissue (8, 16, 32 h), no significant changes were seen in ET A receptor affinity ( K d ) or number B max ): h = 309 ± 75, 225 pM 173 42, 185 17 fmol/mg protein, respectively. Autoradiographic sites for densely expressed on bronchiolar smooth muscle parenchyma with moderate epithelium blood vessels. Histologic sections showed deterioration structures expressing sites. Hence the is stable rat up to post-mortem.