Rapid postmortem gut autolysis in infant rats: a potential problem for investigators.

摘要: Experiments were designed to determine the rate and nature of postmortem autolysis in gut neonatal rats, as necessary baseline information for developing a model human necrotizing enterocolitis. We studied 60 animals, including 33 Wistar 18 Sprague-Dawley rats nine CD-1 mice. The variables examined included age animals (2 or 14 days) length delay holding temperature (20 degrees C 37 C) after sacrifice. At necropsy, bowel was rapidly removed fixed histopathological examination. In all instances, immediately sacrifice normal whereas delays short 30 minutes it abnormal (P less than 0.001), becoming markedly so minutes. prominent features detachment lysis mucosal epithelial cells. not altered day old carcasses held at 20 C. Investigators injury syndromes young should be aware that studies will valid only if specimens room are within 15 death.