An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests.

摘要: This paper proposes a new yet efficient method allowing significant improvement in the on-line analysis of biological cell growing and evolution. The procedure is based on an empirical-mathematical approach for calibration fitting any cell-electrode electrical model. It valid can be extrapolated type cellular line used cell-substrate impedance spectroscopy (ECIS) tests. Parameters bioimpedance model, acquired from ECIS experiments, vary each line, which makes obtaining results difficult and-to some extent-renders them inaccurate. We propose initial characterization, carry out subsequent experiments with same to percentage well filling density (or number well). To perform our technique, so-called oscillation-based test (OBT) employed density. Calibration are validated by performing other different concentrations measurement technique. Accordingly, model determined, further experiment leading more precise electrode-cell system. Furthermore, parameters calculated also techniques. Promising experimental outcomes three cell-lines have been achieved, supporting usefulness this