The Bio-Oscillator: A Circuit for Cell-Culture Assays
作者： Gloria Huertas , Andres Maldonado , Alberto Yufera , Adoracion Rueda , Jose Luis Huertas
DOI: 10.1109/TCSII.2014.2386233
关键词:
摘要: A system for cell-culture real-time monitoring using an oscillation-based approach is proposed. The transforms a cell culture under test into suitable “biological” oscillator, without needing complex circuitry excitation and measurement. obtained oscillation parameters are directly related to biological test, owed empirically extracted cell–electrode electrical model. discrete prototype proposed experimental results with living presented, achieving the expected performances.
sci-hub.se 参考文章(10)
David A. Borkholder, Cell based biosensors using microelectrodes ,(1998)
Gloria Huertas Sánchez, José Luis Huertas Díaz, Diego Vázquez García de la Vega, Adoración Rueda Rueda, Oscillation-Based Test in Mixed-Signal Circuits ,(2006)
Paula Daza, Alberto Olmo, Daniel Cañete, Alberto Yúfera, Monitoring living cell assays with bio-impedance sensors Sensors and Actuators B: Chemical. ,vol. 176, pp. 605- 610 ,(2013) , 10.1016/J.SNB.2012.09.083
P.E. Fleischer, A. Ganesan, K.R. Laker, A switched capacitor oscillator with precision amplitude control and guaranteed start-up IEEE Journal of Solid-state Circuits. ,vol. 20, pp. 641- 647 ,(1985) , 10.1109/JSSC.1985.1052358
Gloria Huertas, Andres Maldonado, Alberto Yufera, Adoracion Rueda, Jose Luis Huertas, Oscillation-Based Test applied to cell culture monitoring ieee sensors. pp. 1- 4 ,(2013) , 10.1109/ICSENS.2013.6688339
Ivar Giaever, Charles R. Keese, Use of Electric Fields to Monitor the Dynamical Aspect of Cell Behavior in Tissue Culture IEEE Transactions on Biomedical Engineering. ,vol. BME-33, pp. 242- 247 ,(1986) , 10.1109/TBME.1986.325896
Jakob Müller, Christian Thirion, Michael W. Pfaffl, Electric cell-substrate impedance sensing (ECIS) based real-time measurement of titer dependent cytotoxicity induced by adenoviral vectors in an IPI-2I cell culture model Biosensors and Bioelectronics. ,vol. 26, pp. 2000- 2005 ,(2011) , 10.1016/J.BIOS.2010.08.075
J Wissenwasser, M J Vellekoop, R Heer, Signal Generator for Wireless Impedance Monitoring of Microbiological Systems IEEE Transactions on Instrumentation and Measurement. ,vol. 60, pp. 2039- 2046 ,(2011) , 10.1109/TIM.2011.2113127
X. Huang, D. Nguyen, D.W. Greve, M.M. Domach, Simulation of microelectrode impedance changes due to cell growth IEEE Sensors Journal. ,vol. 4, pp. 576- 583 ,(2004) , 10.1109/JSEN.2004.831302
Arun Manickam, Aaron Chevalier, Mark McDermott, Andrew D. Ellington, Arjang Hassibi, A CMOS Electrochemical Impedance Spectroscopy (EIS) Biosensor Array IEEE Transactions on Biomedical Circuits and Systems. ,vol. 4, pp. 379- 390 ,(2010) , 10.1109/TBCAS.2010.2081669