Oscillation-Based Test applied to cell culture monitoring
作者： Gloria Huertas , Andres Maldonado , Alberto Yufera , Adoracion Rueda , Jose Luis Huertas
DOI: 10.1109/ICSENS.2013.6688339
关键词:
摘要: A method for cell-culture real-time monitoring by means of Oscillation-Based Test (OBT) technique is proposed. The idea inspired in previous works from the authors area testing analogue integrated circuits and deals with solving some critical points this kind biological measurements. simple topology based on a non-linear element feedback loop employed converting Cell-Culture Under (CCUT) into suitable “biological” oscillator. Then, oscillator parameters (frequency, amplitude, phase, etc...) are used as empirical markers to carry out an appropriate interpretation terms cell size identification, counting, growth, etc. Describing Function (DF) approach employed, involved mathematical calculations analysis “biological circuit”, predicting frequency amplitude oscillations. precise values oscillation closely related cell-electrode overlap cell-culture.
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