Purification of his-tagged proteins by immobilized chelate affinity chromatography: the benefits from the use of organic solvent.
作者： Kees L.M.C. Franken , Hoebert S. Hiemstra , Krista E. van Meijgaarden , Yanri Subronto , J. den Hartigh
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摘要: … to the removal of proteins that aspecifically adhere to the beads or to the tagged protein, this … Finally, we show that proteins are able to refold correctly after isopropanol treatment. Thus, …
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