Survivability, Partitioning, and Recovery of Enveloped Viruses in Untreated Municipal Wastewater
作者： Yinyin Ye , Robert M. Ellenberg , Katherine E. Graham , Krista R. Wigginton
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摘要: Many of the devastating pandemics and outbreaks 20th 21st centuries have involved enveloped viruses, including influenza, HIV, SARS, MERS, Ebola. However, little is known about presence fate viruses in municipal wastewater. Here, we compared survival partitioning behavior two model (MHV ϕ6) nonenveloped bacteriophages (MS2 T3) raw wastewater samples. We showed that MHV ϕ6 remained infective on time scale days. Up to 26% adsorbed solid fraction 6% viruses. Based this behavior, assessed optimized methods for recovering from Our ultrafiltration method resulted mean recoveries (±SD) 25.1% (±3.6%) 18.2% (±9.5%) ϕ6, respectively, 55.6% (±16.7%) 85.5% (±24.5%) MS2 T3, respectively. A ma...
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F M Wellings, A L Lewis, C W Mountain, Demonstration of solids-associated virus in wastewater and sludge. Applied and Environmental Microbiology. ,vol. 31, pp. 354- 358 ,(1976) , 10.1128/AEM.31.3.354-358.1976
R L Ward, D R Knowlton, P E Winston, Mechanism of inactivation of enteric viruses in fresh water. Applied and Environmental Microbiology. ,vol. 52, pp. 450- 459 ,(1986) , 10.1128/AEM.52.3.450-459.1986
T W Hejkal, F M Wellings, A L Lewis, P A LaRock, Distribution of viruses associated with particles in waste water. Applied and Environmental Microbiology. ,vol. 41, pp. 628- 634 ,(1981) , 10.1128/AEM.41.3.628-634.1981
Julian Leibowitz, Gili Kaufman, Pinghua Liu, Coronaviruses: Propagation, Quantification, Storage, and Construction of Recombinant Mouse Hepatitis Virus Current Protocols in Microbiology. ,vol. 21, ,(2011) , 10.1002/9780471729259.MC15E01S21
Rebecca S. Moore, Dene H. Taylor, Lawrence S. Sturman, Michael M. Reddy, G. Wolfgang Fuhs, Poliovirus Adsorption by 34 Minerals and Soils Applied and Environmental Microbiology. ,vol. 42, pp. 963- 975 ,(1981) , 10.1128/AEM.42.6.963-975.1981
G D Lewis, T G Metcalf, Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples. Applied and Environmental Microbiology. ,vol. 54, pp. 1983- 1988 ,(1988) , 10.1128/AEM.54.8.1983-1988.1988
K. R. Wigginton, Y. Ye, R. M. Ellenberg, Emerging investigators series: the source and fate of pandemic viruses in the urban water cycle Environmental Science: Water Research & Technology. ,vol. 1, pp. 735- 746 ,(2015) , 10.1039/C5EW00125K
R B Couch, T R Cate, R G Douglas, P J Gerone, V Knight, Effect of route of inoculation on experimental respiratory viral disease in volunteers and evidence for airborne transmission. Bacteriological Reviews. ,vol. 30, pp. 517- 529 ,(1966) , 10.1128/BR.30.3.517-529.1966
C J Hurst, C P Gerba, I Cech, Effects of environmental variables and soil characteristics on virus survival in soil. Applied and Environmental Microbiology. ,vol. 40, pp. 1067- 1079 ,(1980) , 10.1128/AEM.40.6.1067-1079.1980
L S Sturman, C S Ricard, K V Holmes, Conformational change of the coronavirus peplomer glycoprotein at pH 8.0 and 37 degrees C correlates with virus aggregation and virus-induced cell fusion. Journal of Virology. ,vol. 64, pp. 3042- 3050 ,(1990) , 10.1128/JVI.64.6.3042-3050.1990