作者: Francois Mariotti , Maria E Pueyo , Daniel Tome , Sylvain Mahé , Serge Bérot
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摘要: Pulse seed proteins such as those found in peas (Pisum sativum) contain fractions of very dissimilar composition and properties, which may therefore be differently utilized by the human body. To analyze nutritional value soluble protein pea seed, volunteers ingested a mixed meal 30 g raw purified either [15N]-globulins (G, n = 9) or mix [15N]-albumins (GA, 7) their natural proportions (22:8). Dietary endogenous nitrogen fluxes at terminal ileum were assessed using tube perfusion technique with an isotopic dilution method. Systemic dietary amino acid availability retention acids determined 15N enrichment plasma deamination products blood urine for 8 h postprandially. The results showed that albumin fraction had following effects: 1) significantly lowered real ileal digestibility (94 +/- 2.5% G vs. 89.9 4% GA), probably because direct effect trypsin inhibitors; 2) did not promote acute intestinal losses nitrogen; 3) improve postprandial biological (76.5 3.9% 78.7 3.6% despite fact it corrected globulin deficiency sulfur acids. We conclude both GA are good humans show cysteine-rich albumins have far more modest on efficiency utilization than would expected from scores.