Identification of Dietary and Endogenous Ileal Protein Losses in Pigs by Immunoblotting and Mass Spectrometry
作者： Maud Le Gall , Laurence Quillien , Jacques Guéguen , Hélène Rogniaux , Bernard Sève
关键词: Albumin 、 Pea protein 、 Lectin 、 Biochemistry 、 Biology 、 Vicilin 、 Small intestine 、 Digestion 、 Legumin 、 Storage protein
摘要: Ileal flows and the endogenous or dietary origin of soluble proteins present in ileal digesta were determined pigs fed diets containing different pea cultivars (Solara, Madria Eiffel) micro-ground peas (c.v. Solara). analyzed by electrophoresis densitometry analysis identified LC-MS-MS spectrometry immunoblotting. The differed (P < 0.1) among 3 cultivars; flow Madria-containing diet was higher than that Eiffel- Solara-containing diets. reduced micro-grinding peas. true digestibility losses not correlated. However, at this intestinal level, protein essentially (enzymes, antibodies), from partly digested albumin fraction. Pea lectin PA1 b totally resistant to gastric small digestion a minor peptide PA2 detectable. In contrast, storage proteins, legumin vicilin, detectable antibodies LC-MS-MS.
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