Detection and quantification of Cryptosporidium in HCT-8 cells and human fecal specimens using real-time polymerase chain reaction.

摘要: Cryptosporidium is a significant cause of diarrheal illness worldwide, especially among children and immunocompromised patients. Currently used diagnostic techniques are time-consuming, require skilled technicians, not useful for quantification oocysts in fecal environmental samples. In this study, we examined the use real-time polymerase chain reaction (PCR) assay detecting quantifying parvum three distinct progressively more complex matrices: phosphate-buffered saline (PBS), HCT-8 cells (human ileocecal carcinoma), human specimens. A reliable standard curve was generated using PBS samples spiked with pure oocysts, oocyst starting quantities were calculated infected cell The detected infected/spiked ≥103 oocysts/sample both C. hominis clinical This variety research laboratory will likely prove to be tool laboratory.