Comprehensive analysis of oxylipins in human plasma using reversed-phase liquid chromatography-triple quadrupole mass spectrometry with heatmap-assisted selection of transitions

摘要: Oxylipins, a subclass of lipid mediators, are metabolites various polyunsaturated fatty acids with crucial functions in regulation systemic inflammation. Elucidation their roles pathological conditions requires accurate quantification levels biological samples. We refined an ultra-performance liquid chromatography-multiple reaction monitoring-mass spectrometry (UPLC-MRM-MS)-based workflow for comprehensive and specific 131 endogenous oxylipins human plasma, which we optimized LC mobile phase additives, column, gradient conditions. employed heatmap-assisted strategy to identify unique transitions improve the assay selectivity solid extraction procedures achieve better analyte recovery. The method was validated according FDA guidelines. Overall, 94.4% 95.7% analytes at tested concentrations were within acceptable accuracy (80–120%) precision (CV   0.99. also using standard reference material—SRM 1950 frozen plasma demonstrate inter-lab compatibility.