Cathepsin B expression in colorectal carcinomas correlates with tumor progression and shortened patient survival.

摘要: Cathepsin B is a lysosomal cysteine proteinase that has the ability to degrade several extracellular matrix components at both neutral and acidic pH been implicated in progression of human rodent tumors. We have studied expression cathepsin colorectal tissues using monospecific polyclonal rabbit antibody raised against liver B. In immunoblots normal neoplastic this specifically recognized only 101 cases formalin-fixed, paraffin-embedded tissue (15 mucosa, 17 adenomas, 69 carcinomas). Epithelial cells mucosa adenomas were either negative or showed weak granular reactivity located paranuclear apical cytoplasm superficial cells. Small clusters histiocytes also positive region area lamina propria. carcinomas, increased correlated with advanced stage disease. Increased immunoreactivity malignant was associated diffuse cytoplasmic staining polarized basal pole This contrast punctate pattern observed colonic mucosal tumor stromal cells, enzyme progression. Expression high levels epithelial significantly shorter survival patients. conclusion, our results indicate up-regulated carcinomas compared correlates