Enhanced immunofluorescence detection of a protein marker using a PAA modified ZnO nanorod array-based microfluidic device.

摘要: Zinc oxide (ZnO) often serves as protein microarray substrates owing to its outstanding fluorescence enhancement effect. However, the integration of functional with microfluidic technology detect cancer biomarkers still needs be optimized and promoted, for example, optimization micro/nanostructure hydrophilic modification strategies immunoassays. Here, ZnO nanorod arrays were constructed on inner wall glass capillaries through a chemical method, electrostatic layer by self-assembly was applied modify array polyelectrolyte-polyacrylic acid (PAA). The effects flow rate reagent concentration morphology investigated. array-based capillary, prepared at 25 μL min−1 4 min 50 mM Zn2+ in solution, showed remarkable performance. In addition, introduction PAA suppressed interference nonspecific improved antibody loading capacity effectively. detection carcinoembryonic antigen, limit reached 100 fg mL−1, which indicated that ZnO@PAA device exhibits performance towards markers possesses potential point-of-care diagnostics high throughput biomarker detection.