Activated Proliferation of B-Cell Lymphomas/Leukemias with the SHP1 Gene Silencing by Aberrant CpG Methylation
作者： Maho Koyama , Takashi Oka , Mamoru Ouchida , Yoko Nakatani , Ritsuo Nishiuchi
DOI: 10.1097/01.LAB.0000106503.65258.2B
关键词:
摘要: Previously we showed reduced protein and mRNA expression of the SHP1 gene in lymphoma/leukemia cell lines patient specimens by Northern blot, RT-PCR, Western immunohistochemical analyses. In this study, aberrant methylation promoter was detected many B-cell leukemia/lymphoma as well specimens, including diffuse large lymphoma (methylation frequency 93%), MALT (82%), mantle (75%), plasmacytoma (100%) follicular (96%) methylation-specific PCR, bisulfite sequencing, restriction enzyme-mediated PCR The significantly higher high-grade cases than low-grade (70%), which correlated with no (80%) (54%). It suggests that silencing CpG relates to progression. recovered after treatment demethylating reagent: 5-aza-2'-deoxycytidine. Transfection intact hematopoietic cultured cells, show gene, induced growth inhibition, indicating plays an important role get advantage malignant cells. extraordinarily high (75 100%) indicates is one critical events onset lymphomas/leukemias implications for diagnostic or prognostic markers target therapy. These data support possibility tumor suppressor genes.
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