Fine genetic mapping of greenbug aphid-resistance gene Gb3 in Aegilops tauschii
作者： Perumal Azhaguvel , Jackie C Rudd , Yaqin Ma , Ming-Cheng Luo , Yiqun Weng
DOI: 10.1007/S00122-011-1728-Z
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摘要: The greenbug, Schizaphis graminum (Rondani), is an important aphid pest of small grain crops especially wheat (Triticum aestivum L., 2n = 6x 42, genomes AABBDD) in many parts the world. greenbug-resistance gene Gb3 originated from Aegilops tauschii Coss. (2n 2x 14, genome DtDt) has shown consistent and durable resistance against prevailing greenbug biotypes fields. We previously mapped a recombination-rich, telomeric bin chromosome arm 7DL. In this study, high-resolution genetic mapping was carried out using F2:3 segregating population derived two Ae. accessions, resistant PI 268210 (original donor hexaploid germplasm line ‘Largo’) susceptible AL8/78. Molecular markers were developed by exploring bin-mapped RFLPs, SSRs, ESTs physical map (BAC contigs). Wheat EST BAC end sequences located deletion 7DL3-0.82–1.00 used to design STS (sequence tagged site) or CAPS (Cleaved Amplified Polymorphic Sequence) markers. Forty-five PCR-based chromosomal region spanning locus. now delimited interval 1.1 cM molecular (HI067J6-R HI009B3-R). This localized with closely linked lays solid foundation for based cloning marker-assisted selection breeding.
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