AFLP and STS tagging of a major QTL for Fusarium head blight resistance in wheat.
作者： P.-G. Guo , G.-H. Bai , G. Shaner
DOI: 10.1007/S00122-002-1129-4
关键词:
摘要: Large-scale field screening for Fusarium head blight (FHB) resistance in wheat is difficult because environmental factors strongly influences the expression of genes. Marker-assisted selection (MAS) may provide a powerful alternative. Conversion amplified fragment length polymorphism (AFLP) markers into sequence-tagged site (STS) can generate breeder-friendly MAS. In previous study, one major quantitative trait locus (QTL) on chromosome 3BS was identified by using EcoRI-AFLP and recombinant inbred population derived from cross Ning 7840/Clark. Further mapping with PstI-AFLPs five that were significantly associated QTL. Three them individually explained 38% to 50% phenotypic variation FHB resistance. Two (pAGT/mCTG57, pACT/mCTG136) linked QTL coupling, another (pAG/mCAA244) repulsion. Successful conversion AFLP marker yielded co-dominant STS explains about population. The validated 14 other cultivars first converted an marker.
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