Transcriptome characterization by RNA-Seq reveals the involvement of the complement components in noise-traumatized rat cochleae

摘要: Abstract Acoustic trauma, a leading cause of sensorineural hearing loss in adults, induces complex degenerative process the cochlea. Although previous investigations have identified multiple stress pathways, comprehensive analysis cochlear responses to acoustic injury is still lacking. In current study, we used next-generation RNA-sequencing (RNA-Seq) technique sequence whole transcriptome normal and noise-traumatized sensory epithelia (CSE). CSE tissues were collected from rat inner ears 1 d after rats exposed 120-dB (sound pressure level) noise for 2 h. The RNA-Seq generated over 176 million reads 164 CSE. Alignment these sequences with Rn4 genome revealed expression 17,000 gene transcripts CSE, 2000 which exclusively expressed either or Seventy-eight differentially (70 upregulated 8 downregulated) trauma. Many genes are related innate immune system. Further analyses using quantitative real time PCR confirmed constitutive complement organ Corti changes levels factor I (Cfi) component 1, s subcomponent (C1s) Moreover, protein strong Cfi C1s proteins Corti. Importantly, exhibited following Collectively, results investigation suggest involvement components