Library Preparation and Data Analysis Packages for Rapid Genome Sequencing
作者： Kyle R. Pomraning , Kristina M. Smith , Erin L. Bredeweg , Lanelle R. Connolly , Pallavi A. Phatale
DOI: 10.1007/978-1-62703-122-6_1
关键词:
摘要: High-throughput sequencing (HTS) has quickly become a valuable tool for comparative genetics and genomics is now regularly carried out in laboratories that are not connected to large centers. Here we describe an updated version of our protocol constructing single- paired-end Illumina libraries, beginning with purified genomic DNA. The present can also be used "multiplexing," i.e. the analysis several samples single flowcell lane by generating "barcoded" or "indexed" libraries way independent from Illumina-supported methods. To analyze results, suggest approaches but end users should aware this evolving field currently many alignment (or "mapping") counting algorithms being developed tested.
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