Bulk Segregant Analysis by High-Throughput Sequencing Reveals a Novel Xylose Utilization Gene from Saccharomyces cerevisiae

摘要: Fermentation of xylose is a fundamental requirement for the efficient production ethanol from lignocellulosic biomass sources. Although they aggressively ferment hexoses, it has long been thought that native Saccharomyces cerevisiae strains cannot grow fermentatively or non-fermentatively on xylose. Population surveys have uncovered few naturally occurring are weakly xylose-positive, and some S. genetically engineered to xylose, but no strain, either natural engineered, yet reported as efficiently glucose. Here, we used medium-throughput screen identify can increase in optical density when presented sole carbon source. We identified 38 this utilization phenotype, including cerevisiae, other sensu stricto members, hybrids between them. All xylose-utilizing wine yeasts, those could produce meiotic progeny, phenotype segregates single gene trait. mapped by Bulk Segregant Analysis (BSA) using tiling microarrays high-throughput sequencing. The putative xylitol dehydrogenase, which name XDH1, located subtelomeric region right end chromosome XV not present S288c reference genome. further characterized performing expression dissecting endogenous pathway. demonstrated yeasts capable utilizing source, genetic basis trait well pathway, feasibility BSA