Functional Analyses of Trichoderma reesei LAE1 Reveal Conserved and Contrasting Roles of This Regulator

摘要: The putative methyltransferase LaeA is a global regulator that affects the expression of multiple secondary metabolite gene clusters in several fungi, and it can modify heterochromatin structure Aspergillus nidulans. We have recently shown ortholog Trichoderma reesei (LAE1), fungus an industrial producer cellulase hemicellulase enzymes, regulates cellulases polysaccharide hydrolases. To learn more about function LAE1 T. reesei, we assessed effect deletion overexpression lae1 on genome-wide expression. found addition to positively regulating 7 17 polyketide or nonribosomal peptide synthases, genes encoding ankyrin-proteins, iron uptake, heterokaryon incompatibility proteins, PTH11-receptors, oxidases/monoxygenases are major categories also regulated by LAE1. chromatin immunoprecipitation sequencing with antibodies against histone modifications known be associated transcriptionally active (H3K4me2 -me3) silent (H3K9me3) detected 4089 bearing one these methylation marks, which 75 exhibited correlation between either H3K4me2 H3K4me3 regulation Transformation laeA-null mutant A. nidulans did not rescue sterigmatocystin formation further impaired sexual development. interact VeA yeast two-hybrid assays, whereas interacted ortholog, VEL1. was required for vel1, orthologs velB VosA unaffected deletion. Our data show biological roles much less conserved than hitherto thought. In appears predominantly regulate increasing relative fitness its environment.