Regulation of secondary metabolite production in the fungal tomato pathogen Cladosporium fulvum

摘要: Cladosporium fulvum is a non-obligate biotrophic fungal tomato pathogen for which fifteen secondary metabolite (SM) gene clusters were previously identified in its genome. However, most of these SM biosynthetic pathways remain cryptic during growth planta and different vitro conditions. The sole produced the pigment cladofulvin. In this study, we attempted to activate order identify new compounds by C. fulvum. For purpose, manipulated orthologues global regulators VeA, LaeA HdaA known regulate biosynthesis other species. fulvum, deleting or over-expressing yielded no detectable SMs. Yet, quantification cladofulvin revealed that CfHdaA an activator whilst CfVeA CfLaeA seemed act as repressors production. wild type strain, was affected carbon source, with highest production under limitation traces only presence saccharose. Repression saccharose dependent on both CfLaeA. Deletion caused sterile mycelia, Δcfhdaa deletion mutants sporulated, suggesting not linked asexual reproduction. Profiling transcription showed CfHdaA-mediated regulation independent Our data suggest directly affects effect indirect, role outside Velvet complex. conclusion, our results from fungi indicate manipulation universal tool discover natural products.