LaeA negatively regulates dothistromin production in the pine needle pathogen Dothistroma septosporum.

摘要: In filamentous fungi both pathway-specific and global regulators regulate genes involved in the biosynthesis of secondary metabolites. LaeA is a regulator that was named for its mutant phenotype, loss aflR expression, due to effect on aflatoxin-pathway AflR Aspergillus spp. The pine needle pathogen Dothistroma septosporum produces polyketide virulence factor, dothistromin, chemically related aflatoxin whose pathway are also regulated by an ortholog AflR. However, dothistromin distinctive because it switched during early (rather than late) exponential growth phase dispersed six loci across one chromosome instead being clustered. It therefore interest determine whether function conserved D. septosporum. To address this question, (DsLaeA) identified analyzed contrast production spp., deletion DsLaeA resulted enhanced increased expression regulatory gene DsAflR. Although other putative metabolite showed range different responses function, thin layer chromatography revealed levels previously unknown mutants. addition, these mutants exhibited reduced asexual sporulation, germination hydrophobicity. Our data suggest although developmental role conserved, regulation metabolism differs from A. nidulans appears be species specific. This study provides step towards understanding fundamental differences clustered fragmented groups may shed light functional adaptation metabolism.