MoLAEA Regulates Secondary Metabolism in Magnaporthe oryzae

摘要: ABSTRACT Fungi are rich sources of secondary metabolites pharmaceutical importance, such as antibiotics, antitumor agents, and immunosuppressants, well harmful toxins. Secondary play important roles in the development pathogenesis fungi. LaeA is a global regulator metabolism was originally reported Aspergillus nidulans; however, its role Magnaporthe oryzae has not yet been reported. Here, we investigated gene homologous to LAEA (loss AflR expression) spp. oryzae, named M. oryzaeLAEA (MoLAEA). Studies on MoLAEA overexpression knockdown strains have suggested that this acts negative sporulation melanin synthesis. However, it involved growth oryzae. Transcriptomic data indicated regulated genes metabolism. Interestingly, observed (for first time, our knowledge) benzylpenicillin (penicillin G) synthesis Overexpression increased penicillin G production, whereas silenced strain showed complete absence compared presence wild type. We also MoLaeA interacted with MoVeA, velvet family protein fungal metabolism, nucleus. This study though may make any contribution rice blast pathogenesis, regulates thus can be further studied for identifying other new uncharacterized fungus. IMPORTANCEM. causes disease, most serious disease cultivated affecting production. The genome shown number but them uncharacterized. In fact, studies filamentous fungi, hardly work done It here being synthesized process. step understanding biosynthesis pathway unraveled details how works by forming nuclear complex MoVeA indicating functional conservation across All these findings open up avenues more relevant investigations genetic regulation