Genome-Based Cluster Deletion Reveals an Endocrocin Biosynthetic Pathway in Aspergillus fumigatus

摘要: Endocrocin is a simple anthraquinone frequently identified in extracts of numerous fungi. Several biosynthetic schemes for endocrocin synthesis have been hypothesized, but to date, no dedicated secondary metabolite gene cluster that produces this polyketide as its major has identified. Here we describe our and regulatory characterization the Aspergillus fumigatus. This first report species. The genes required production are regulated by global regulator metabolism, LaeA, encode an iterative nonreducing synthase (encA), physically discrete metallo-β-lactamase type thioesterase (encB), monooxygenase (encC). Interestingly, deletion immediately adjacent encC, termed encD encoding putative 2-oxoglutarate-Fe(II) oxidoreductase, resulted higher levels than wild-type strain, whereas overexpression eliminated accumulation. We found encA transcript encA-D endocrocin. discuss model enc one evolutionary origin fungal anthraquinones derived from metallo-β-lactamase-type thioesterase.