Co-ordination between BrlA regulation and secretion of the oxidoreductase FmqD directs selective accumulation of fumiquinazoline C to conidial tissues in Aspergillus fumigatus.

摘要: Summary Aerial spores, crucial for propagation and dispersal of the Kingdom Fungi, are commonly initial inoculum pathogenic fungi. Natural products (secondary metabolites) have been correlated with fungal spore development enhanced virulence in human pathogen Aspergillus fumigatus but mechanisms metabolite deposition unknown. Metabolomic profiling A. fumigatus deletion mutants fumiquinazoline (Fq) cluster genes reveal that first two Fq cluster, FqF FqA, produced to comparable levels all tissues final enzymatically derived product, FqC, predominantly accumulates spore. Loss sporulation-specific transcription factor, BrlA, yields a strain unable produce FqA or FqC. Fluorescence microscopy showed FmqD, oxidoreductase required generate was secreted via Golgi apparatus cell wall an actin-dependent manner. In contrast, other members pathway including putative transporter, FmqE – which had no effect on biosynthesis were internal hyphae. The co-ordination BrlA-mediated tissue specificity FmqD secretion presents previously undescribed mechanism direct localization specific secondary metabolites spores differentiating fungus.