Secondary Metabolism and Biotrophic Lifestyle in the Tomato Pathogen Cladosporium fulvum

摘要: Cladosporium fulvum is a biotrophic fungal pathogen that causes leaf mould of tomato. Analysis its genome suggested high potential for production secondary metabolites (SM), which might be harmful to plants and animals. Here, we have analysed in detail the predicted SM gene clusters C. employing phylogenetic comparative genomic approaches. Expression core genes was measured by RT-qrtPCR produced SMs were determined LC-MS NMR analyses. The contains six are conserved other species, undergone rearrangements losses associated with presence transposable elements. Although being biotroph, has produce elsinochrome cercosporin toxins. However, corresponding not expressed during infection Only two genes, PKS6 NPS9, show expression planta, but both significantly down regulated colonization mesophyll tissue. In vitro profiling detected only one major compound identified as cladofulvin. likely involved this pigment because it under these conditions. Cladofulvin does cause necrosis on Solanaceae any antimicrobial activity. contrast fungi reduced capacity, our studies suggest down-regulation biosynthetic pathways represent another mechanism lifestyle.