Porcine Invariant Natural Killer T Cells: Functional Profiling and Dynamics in Steady State and Viral Infections.

摘要: Pigs are important livestock and comprehensive understanding of their immune responses in infections is critical to improve vaccines therapies. Moreover, similarities between human swine physiology suggest that pigs a superior animal model for immunological studies. However, paucity experimental tools systematic analysis the represent major disadvantage. To evaluate pig as biomedical additionally expand knowledge rare cell populations swine, we established multicolor flow cytometry platform surface marker expression cellular porcine invariant Natural Killer T cells (iNKT). In humans, iNKT among first line defenders various tissues, respond CD1d-restricted antigens become rapidly activated. Naive were CD3+/CD4-/CD8+ or CD3+/CD4-/CD8- displayed an effector- memory-like phenotype (CD25+/ICOS+/CD5hi/CD45RA-/CCR7 ± /CD27+). Based on transcription factors bet cell-specific promyelocytic leukemia zinc finger protein (PLZF), differentiated into functional subsets. Analogous cells, vitro stimulation leukocytes with CD1d ligand α-galactosylceramide resulted rapid proliferation, evidenced by increase frequency Ki-67 expression. this approach revealed CD25, CD5, ICOS, histocompatibility complex class II (MHC II) activation markers cells. Activated also expressed interferon-γ, upregulated perforin expression, degranulation. steady state, was highest newborn piglets decreased age. Upon infection two viruses high relevance expanded. Animals infected African fever virus peripheral blood, regional lymph nodes, lungs. During Influenza A infection, percentage increased lung broncho-alveolar lavage. Our in-depth characterization contributes better responses, thereby facilitating design innovative interventions against infectious diseases. provide new evidence endorses suitability research.