Electroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564

摘要: The exposure of bacterial cells to pulsed electric fields (PEF) leads the reversible formation pores in cell membrane if an applied energy is below critical level. Therefore, effect field pulses with amplitudes 14 kV/cm and up 12.2 J/cm(3) on growth Lactobacillus plantarum 564 was investigated. After PEF treatments, lactobacilli De Man-Rogosa-Sharpe broth at 37 degrees C monitored by isothermal calorimetry, absorbance plate counts. All treatments resulted a higher rate PEF-treated during early mid-log phase, especially samples treated lower intensities (1.3-5.5 J/cm(3)). transport ions molecules through (which facilitates electroporated lactobacilli) particularly evident mid-exponential where doubling time reduced more than 3 times after 5.5 Yore. heat production also higher, indicating enhanced metabolic activity cells. Moreover, had better acidification ability untreated ones. It can be summarized that input 12 potentially induce electroporation membrane, which has positive impact lactobacilli. (Less)