Prophylactic properties of a Leishmania-specific hypothetical protein in a murine model of visceral leishmaniasis.

摘要: In this work, the effect of vaccination a newly described Leishmania infantum antigenic protein has been studied in BALB/c mice infected with parasite species. The LiHyD was characterized after proteomic screening performed sera from dogs suffering visceral leishmaniasis (VL). Its recombinant version expressed, purified and administered to combination saponin. As result 10 weeks challenge using an infective dose L. stationary promastigotes, vaccinated showed lower burdens different organs (liver, spleen, bone marrow footpads' draining lymph nodes) than inoculated adjuvant alone or vaccine diluent. Protected anti-Leishmania IgG2a antibodies predominant IL-12-driven IFN-γ production (mainly produced by CD4(+) T cells) against proteins, whereas unprotected controls IgG1 parasite-mediated IL-4 IL-10 responses. Vaccinated anti-LiHyD humoral response, their spleen cells were able secrete LiHyD-specific IFN-γ, IL-12 GM-CSF cytokines before infection. protection correlated Leishmania-specific on nitric oxide. Altogether, results indicate that new could be considered formulations VL.